Technology

Computer vision that reveals the detail in microbial specimens

PhAST’s internally developed computer vision resolves individual bacteria among other cells and debris, with no isolation and no staining required. The analysis works from distributions of measurements taken across many individual cells, exposing details of the early stages of antimicrobial interaction. A locked machine learning pipeline converts the complex cellular response to antimicrobials into a categorical output.

PhAST builds with interpretability as a priority at all stages. It is what allows us to develop robust assays: to trace the cause of an error, and to recognize a sample outside the range the analysis was trained on.

PhAST believes this approach is a foundation for diagnostics well beyond our first assay: computer vision strong enough to characterize the form and behavior of cells in conditions close to their original specimen.

Single-cell level analysis

Single cell analysis
Computer vision example. E. coli from a positive blood culture sample after 80 minutes of exposure to ceftriaxone, with bacterial objects segmented and identified. Colored outlines illustrate a subset of the object types used for AST. The display represents a small fraction of the area imaged.

Early phenotypic response

Early phenotypic response
Raw data illustration. E. coli reference strains exposed to ceftazidime, alongside untreated controls from the same cartridge. At 80 minutes after exposure, the susceptible strain’s response to the antimicrobial is apparent by eye. The displays represent a small fraction of each area imaged.